ASSESSMENT OF THE EFFECTS OF TEMPERATURE AND THERMAL TREATMENT DURATION ON THE STABILITY OF PHENOLIC CONSTITUENTS IN SWEET EXTRACTS PRODUCED FROM RARA NEAGRĂ GRAPE MARC
DOI:
https://doi.org/10.52326/jes.utm.2026.33(2).08Keywords:
grape pomace, Rara Neagră, thermal treatment, anthocyanins, polyphenols, antioxidant compounds, by-product valorization, food processingAbstract
Sweet red grape pomace represents a valuable source of anthocyanins, polyphenols, and flavonoids. Heating affects the content of biologically active compounds. However, wellcontrolled thermal processing can contribute to their preservation. The aim of this study was to provide a detailed presentation of the degradation of biologically active compounds during heating, as well as to describe their thermal behavior. The study was based on the preparation and investigation of hydroalcoholic extracts acidified with citric acid obtained from unfermented sweet red grape pomace of the Rara Neagră grape variety, harvest year 2025. Following thermal treatment within the studied temperature range (80–140°C, 7–30 min), the total phenolic content of the acidified Rara Neagră extracts decreased from 50.67 mg GAE/g DW to 17.98 mg GAE/g DW. The total anthocyanin content remained almost constant. In contrast, the experimental results highlighted a decrease in the content of monomeric anthocyanins. Analysis of the absorption spectra revealed the highest stability at 80–100°C and rapid degradation at 120–140°C. The stability observed at 80–100°C indicates that these compounds can withstand moderate food-processing operations (pasteurization and shortterm thermal treatments). Therefore, the obtained results are of direct importance for the food industry and for the valorization of wine industry by-products.
References
Balea SS, Pârvu AE, Pârvu M, Vlase L, Dehelean CA, Pop TI (2020) Antioxidant, anti-inflammatory, and antiproliferative effects of Vitis vinifera L. var. Fetească Neagra and Pinot Noir pomace extracts. Front. Pharmacol 11:990. https:// doi: 10.3389/fphar.2020.00990
Almanza-Oliveros A, Bautista-Hernandez I, Castro-Lopez C, Aguilar-Zarate P, Meza-Carranco Z, Rojas R, Michel M, Cristian G, Martinez-Avila G (2024) Grape Pomace—Advances in Its Bioactivity, Health Benefits, and Food Applications. Foods 13:580. https:/ / doi: 10.3390/foods13040580
Teresa Abreu T, Luís C, José S, Teixeira J, Rosa Perestrelo R (2025) Unveiling potential functional applications of grape pomace extracts based on their phenolic profiling, bioactivities, and circular bioeconomy. Biomass Conversion and Biorefinery 15:31491–31506. https://doi.org/10.1007/s13399-025-06578-6
Ghendov-Mosanu A, Cojocari D, Balan G, Patras A, Lung I, Soran ML, Opri O, Cristea E, Sturza R (2022) Chemometric Optimization of Biologically Active Compounds Extraction from Grape Marc: Composition and Antimicrobial Activity. Molecules 27(5) :1610. https://doi.org/10.3390/molecules27051610.
Wittenauer J, Mäckle S, Sußmann D, Schweiggert-Weisz U, Carle R (2020) Inhibitory effects of polyphenols from grape pomace extract on collagenase and elastase activity. Fitoterapia 101:179–187. https://doi.org/10.1016/j.fitote.2015.01.005
Karastergiou A, Gancel AL, Jourdes M, Teissedre PL (2024) Valorization of Grape Pomace: A Review of Phenolic Composition, Bioactivity, and Therapeutic Potential. Antioxidants 13(9):1131. https://doi.org/10.3390/antiox13091131
Peixoto CM, Dias MI, Alves MJ, Calhelha RC, Barros L, Pinho SP, Ferreira IC (2018) Grape pomace as a source of phenolic compounds and diverse bioactive properties Food Chemistry 253:132–138. https://doi.org/10.1016/j.foodchem.2018.01.163
Patras A, Brunton NP, O’Donnell C, Tiwari BK (2019) Effect of thermal processing on anthocyanin stability in foods. Trends in Food Science & Technology 21(1): 3–11. https://doi.org/10.1016/j.tifs.2009.07.004.
Dranca F, Oroian M (2019) Kinetic Improvement of Bioactive Compounds Extraction from Red Grape (Vitis vinifera Moldova) Pomace by Ultrasonic Treatment. Foods 8(8): 353. https://doi.org/10.3390/foods8080353
Zekun Z, Dong Y (2019) Economical and eco-friendly isolation of anthocyanins from grape pomace with higher efficiency. Food Chem X 10 15:100419. https://doi.org/10.1016/j.fochx.2022.100419
Tikhonova A, Ageeva N, Globa E (2021) Grape pomace as a promising source of biologically valuable components. In: 2021 BIO Web of Conferences 34: 6002. https://doi.org/10.1051/bioconf/20213406002
Musteață G, Sclifos A, Gherciu-Musteață L,Covaci E (2017) Methodological guide. Techno-chemical and microbiological control of alcoholic beverages. TUM, Chisinau, Republic of Moldova [in Romanian]
Covaci E, Arhip V (2020) Methodological guide. Technological operations for conditioning and stabilizing wines. TUM, Chisinau, Republic of Moldova [in Romanian]
Delić K, Milinčić DD, Pešić MB, Lević S, Nedović VA, Gancel A-L, Jourdes M, Teissedre P-L (2024) Grape, wine and pomace anthocyanins: winemaking biochemical transformations, application and potential benefits. OENO One 58(4). https://doi.org/10.20870/oeno-one.2024.58.4.8039
Serea, D (2023) The valorization of various biologically active compounds from red grapes processing byproducts and the obtainment of certain value-added ingredients. PhD Thesis, Dunărea de Jos University of Galați, Republic of Romania.
Volf I, Ignat I, Neamțu M, Popa VI (2014) Thermal degradation of grape marc polyphenols. Food Chemistry 160: 313–320. https://doi.org/10.1016/j.foodchem.2014.03.080
Oancea S (2021) A Review of the Current Knowledge of Thermal Stability of Anthocyanins and Approaches to Their Stabilization to Heat. Antioxidants 10(9) :1337. https://doi.org/10.3390/antiox10091337
Turturica M (2018) Kinetic Behavior of Anthocyanins from Fruits during Processing in Model and Food Systems. PhD Thesis, Dunărea de Jos University of Galați, Republic of Romania.
Yalçınöz Ş, Erçelebi E (2017) Heat-induced degradation kinetics of monomeric anthocyanins and color of pomegranate (Punica Granatum L.) concentrate. Agriculture & Food 5.ISSN 1314-8591
Khoo H, Azlan A, Tang S, Lim S (2017) Anthocyanidins and anthocyanins: colored pigments as food, pharmaceutical ingredients, and the potential health benefits. Food Nutrition Research 61:1361779. https://doi.org/10.1080/16546628.2017.1361779.
Pereira RN, Coelho MI, Genisheva Z, Fernandes JM, Vicente AA, Pintado ME, Teixeira JA (2020) Using Ohmic Heating effect on grape skins as a pretreatment for anthocyanins extraction. Food and Bioproducts Processing 124:320–328. https://doi.org/10.1016/j.fbp.2020.09.009
Castellanos-Gallo L, Ballinas-Casarrubias L, Espinoza-Hicks J C, Hernández-Ochoa LR, Nayzzel MuñozCastellanos L, Zermeño-Ortega MR, Borrego-Loya A, Salas E (2022) Grape Pomace Valorization by Extraction of Phenolic Polymeric Pigments: A Review. Processes 10(3):469. https://doi.org/10.3390/pr10030469
Gordillo B, Sigurdson GT, Lao F, González-Miret ML, Heredia F J, Giusti MM (2022) Assessment of the color modulation and stability of naturally copigmented anthocyanin-grape colorants with different levels of purification. Food Research International 106:791–799. https://doi.org/10.1016/j.foodres.2018.01.057
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